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ADIAVETTM kits contain only amplification PCR reagents. In order to achieve very good performances on a wide range of samples, we recommend and validate many different DNA/RNA global extraction methods. It is very useful to stay flexible but without decreasing any performances (sensitivity, inhibition rate).
Today DNA and RNA extracts usable with ADIAVETTM kits can be prepared with :
Different technologies of purification : silica membrane, magnetic beads, ultra-filtration
Different automated system : centrifugation, vacuum, magnetic
For different throughputs : from individual analysis to 96 samples extraction per run
For some specific applications we propose our own extraction method with ADIAPURETM range in combination with ADIAVETTM PCR kits :
Samples preparation steps (individual or pool) are developed and validated to be adapted to different epidemiological situations : diagnostic, individual analysis, screening with pool of samples or environmental samples.
ADIAPURE™ kits :
Find out more details on the ADIAPURE™ DNA purification range on the technical details tab.
PERFORMANCE : ADIAPURE™ kits are fully validated with ADIAVETTM Real Time PCR kits and improve their performances (level of inhibition, sensitivity)
EASY TO USE : ADIAPURETM protocols are simple and adapted to a routine buzzy diagnostic laboratory
HIGH THROUGHPUT : Direct lysis method or 96 wells-plate purification system allow to treat a lot of samples all together
In order to achieve very good performances on a wide range of samples, we recommend and validate many different DNA/RNA global extraction methods. It is very useful to stay flexible but without decreasing any performances (sensitivity, inhibition rate).
In this objective also, ADIAPURETM protocols have been developed for some specific veterinary applications where other common DNA extraction methods didn’t give expected results in term of performances and/or throughput.
Kits include all components for DNA extraction and/or purification.
Find out more details on equipment requested for using ADIAPURE™ DNA purification range on the option & accessories tab.
ADIAPURE™ PURIFICATION KIT
Pathogen |
Samples |
Lysis |
Purification |
Storage |
Avian mycoplasmas and Ornithobacterium rhinotracheale |
Swabs (tracheal or tissues). Pooling (up to 6 swabs) |
Proteinase K |
Filtration on 2 filters (96 wells) with a vacuum pump and a manifold |
+2/8°C |
ADIAPURE™ MILK KIT
Pathogen |
Samples |
Lysis |
Purification |
Storage |
Mycobacterium avium subsp.paratuberculosis |
Dairy products (milk and cheese) |
Grinding (glass beads) and proteinase K |
No purification step |
+2/8°C |
ADIAPURE TLB™ BUFFER
Pathogen |
Samples |
Lysis |
Purification |
Storage |
BVD (Bovine Viral Diarrhea virus) |
Ear notches (individual and pooling up to 20 samples) |
ADIAPURETM TLB Buffer |
No purification step |
+2/8°C |
A manifold for 96-wells plates (eg : PALL).
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